An archaeal CRISPR type III-B system exhibiting distinctive RNA targeting features and mediating dual RNA and DNA interference

摘要

CRISPR-Cas systems provide a small RNA-based mechanism to defend against invasive genetic elements in archaea and bacteria. To investigate the in vivo mechanism of RNA interference by two type III-B systems (Cmr-alpha and Cmr-beta) in Sulfolobus islandicus, a genetic assay was developed using plasmids carrying an artificial mini-CRISPR (AC) locus with a single spacer. After pAC plasmids were introduced into different strains, Northern analyses confirmed that mature crRNAs were produced from the plasmid-borne CRISPR loci, which then guided gene silencing to target gene expression. Spacer mutagenesis identified a trinucleotide sequence in the 3'-region of crRNA that was crucial for RNA interference. Studying mutants lacking Cmr-alpha or Cmr-beta system showed that each Cmr complex exhibited RNA interference. Strikingly, these analyses further revealed that the two Cmr systems displayed distinctive interference features. Whereas Cmr-beta complexes targeted transcripts and could be recycled in RNA cleavage, Cmr-alpha complexes probably targeted nascent RNA transcripts and remained associated with the substrate. Moreover, Cmr-beta exhibited much stronger RNA cleavage activity than Cmr-alpha. Since we previously showed that S. islandicus Cmr-alpha mediated transcription-dependent DNA interference, the Cmr-alpha constitutes the first CRISPR system exhibiting dual targeting of RNA and DNA.