SEVERE INSTEAD OF MILD HYPERGLYCEMIA INHIBITS NEUROGENESIS IN THE SUBVENTRICULAR ZONE OF ADULT RATS AFTER TRANSIENT FOCAL CEREBRAL ISCHEMIA

摘要

Accumulated evidence suggests that enhanced neurogenesis stimulated by ischemic injury contributes to stroke outcome. However, it is unclear whether hyperglycemia, which is frequently tested positive in patients with acute ischemic stroke, influences stroke-induced neurogenesis. The aim of the present study is to examine the effect of hyperglycemia on stroke-induced neurogenesis in a rat model of transient focal cerebral ischemia. For this purpose, adult male Sprague-Dawley rats (220-250 g) were subjected to 90 min of middle cerebral artery occlusion (MCAO). Glucose was administered during ischemia to produce target blood levels ranging from 4.83 +/- 0.94 mM (normo-glycemia) to 20.76 +/- 1.56 mM. To label proliferating cells in ischemic ipsilateral subventricular zone (SVZ) of lateral ventricles, 5'-bromo-2'-deoxyuridine (BrdU) was injected 24 h after MCAO. Brains were harvested 2 h post-BrdU to evaluate the effects of hyperglycemia on infarct volume and SVZ cell proliferation. Rats that were severely hyperglycemic (19.26 +/- 1.48 mM to 20.76 +/- 1.56 mM) during ischemia had 24.26% increase in infarct volume (P < 0.05) and more serious neurological function deficits (P < 0.05). The severe hyperglycemic rats also showed dramatically decreased proliferation of neural stem/progenitor cells (NSPCs) (P < 0.05) and down-regulation of the phosphorylation of cyclic-AMP response element-binding protein (pCREB) (P < 0.05) and brain-derived neurotrophic factor (BDNF) (P < 0.05) in ipsilateral SVZ. But the abovementioned detrimental effects were not observed in rats that were rendered with mild hyperglycemia (9.43 +/- 1.39-10.13 +/- 1.24 mM). Our findings indicate that severe instead of mild hyperglycemia exacerbates ischemic injury and inhibits stroke-induced SVZ neurogenesis by a mechanism involving suppression of CREB and BDNF signaling. (C) 2015 IBRO. Published by Elsevier Ltd. All rights reserved.