Anticoagulant Activity and Molecular Docking of 7,9-bis(4-Chlorophenyl)-6-methyl-1,4-dioxa-8-azaspiro[4.5]decane through Target Protein Thrombin

摘要

The present study reported the synthesis and anticoagulant activity of spiro compounds, the potential compound was subjected to study their binding mode and interaction using bioinformatics tools. Among the five compounds tested, 7,9-bis(4-chlorophenyl)-6-methyl-1,4,8-triaza spiro[4.5]decane has prolonged activated partial thromboplastin time and prothrombin time as 22.7s and 18.5s at 25 μg mL~(-1), respectively. Hence, the mandatory way study was carried out for this compound by using bioinformatics method. The interaction results shows that this compound binds in the active site of the target protein thrombin which is similar to that of existing inhibitor and it may also considered as an anticoagulant drug in future.