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High-Performance Graphene-Titania Platform for Detection of Phosphopeptides in Cancer Cells

机译:高性能石墨烯-二氧化钛平台可检测癌细胞中的磷酸肽

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Phosphopeptides play a crucial role in many biological processes and constitute some of the most powerful biomarkers in disease detection. However they are often present in very low concentration, which makes their detection highly challenging. Here, we demonstrate the use of a solution-dispersible graphene-titania platform for the selective extraction of phosphopeptides from peptide mixtures. This is followed by direct analysis by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS). The efficient charge and energy exchange between graphene and TiO_(2) during laser irradiation in SELDI-TOF MS promotes the soft ionization of analytes and affords a detection limit in the attomole range, which is 10~(2)-10~(5) more sensitive than conventional platforms. The graphene-titania platform can also be used for detecting phosphopeptides in cancer cells (HeLa cells), where it shows high specificity (94percent). An expanded library of 967 unique phosphopeptides is detected using significantly reduced loading of extraction matrixes compared to conventional TiO_(2) bead-based assays.
机译:磷酸肽在许多生物学过程中起着至关重要的作用,并构成疾病检测中一些最强大的生物标记。然而,它们通常以非常低的浓度存在,这使其检测具有很高的挑战性。在这里,我们演示了使用溶液可分散的石墨烯-二氧化钛平台从肽混合物中选择性提取磷酸肽。然后通过表面增强的激光解吸/电离飞行时间质谱仪(SELDI-TOF MS)进行直接分析。在SELDI-TOF MS中进行激光辐照期间,石墨烯与TiO_(2)之间的有效电荷和能量交换促进了分析物的软电离,并提供了在attomole范围内的检测极限,即10〜(2)-10〜(5)比传统平台更敏感。石墨烯-二氧化钛平台还可用于检测癌细胞(HeLa细胞)中的磷酸肽,该平台显示出高特异性(94%)。与传统的基于TiO_(2)珠的检测方法相比,使用提取基质的负载明显减少,可以检测到967个独特的磷酸肽的扩展文库。

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