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In situ monitoring system for chlorophyll fluorescence parameters of tomato at greenhouse in northern China.

机译:中国北方温室番茄叶绿素荧光参数原位监测系统

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摘要

Light pattern plays a crucial role in crop photosynthesis in different growth stages, and the photosynthesis process can be reflected by the chlorophyll fluorescence parameters. The chlorophyll fluorescence monitoring system in this study is composed of a PC (host computer), a programmable constant current power supply and a MINI-PAM, which is a modulation fluorescence detector. The chlorophyll fluorescence parameters of tomato, such as Fs (Steady-state fluorescence), F'm (Maximal fluorescence) in illuminated samples, ETR (Electron Transport Rate), PAR (Photosynthetically Active Radiation) and Phi PSII (Quantum yield of PSII during exposure to light) were measured, then the light use efficiency was analyzed according to the measured light response curve of tomato in a fixed growth cycle, and the influence of light intensity on the tomato growth mechanism was determined further. According to the relation model between light intensity and fluorescence parameters, the information of host computer was set to monitor the quantitative fluorescence parameters, so the light control environment was established to guide the dynamic output. The 'Liaoyuanduoli' tomato grown in greenhouse of north China was studied in this experiment. The results show that the change of fluorescent parameters depends on the change of the PAR closely. The increase of PAR makes Fs higher significantly while F'm lower correspondingly, thus the quantum yield of Phi PSII was decreased. Therefore, the system can achieve monitoring in the quantitative fluorescent parameters, so as to realize the actual measurement and quantitative control of plant photosynthetic capacity.
机译:光照模式在不同生育阶段的作物光合作用中起着至关重要的作用,其光合作用过程可以通过叶绿素荧光参数反映出来。本研究中的叶绿素荧光监测系统由PC(主机),可编程恒流电源和MINI-PAM(调制荧光检测器)组成。番茄的叶绿素荧光参数,如稳定样品中的Fs(稳态荧光),F'm(最大荧光),ETR(电子传输速率),PAR(光合有效辐射)和Phi PS II (曝光时PSII的量子产率),然后根据测得的番茄在固定生长周期内的光响应曲线分析光利用效率,并确定光强度对番茄生长机理的影响进一步。根据光强与荧光参数之间的关系模型,设置上位机信息对荧光定量参数进行监控,建立了光控环境以指导动态输出。本实验研究了北方北方温室栽培的“辽源多里”番茄。结果表明,荧光参数的变化与PAR的变化密切相关。 PAR的增加使Fs显着升高,而F'm相应降低,从而降低了Phi PS II 的量子产率。因此,该系统可以实现对荧光定量参数的监控,从而实现对植物光合能力的实际测量和定量控制。

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