Development of micropropagated shoots and plants of caroa in different substrates.

摘要

Caroa (Neoglaziovia variegata) is a native Bromeliaceae of Caatinga used for fiber extraction in the Northeast region of Brazil. The anthropic activity put this species among the threatened ones. The work aimed to determine a substrate adapted for an efficient development of shoots and plants of caroa produced in vitro that were not submitted to the in vitro rooting stage. Shoots without roots and rooting plants (during the multiplication phase) were obtained from two culture media: MS with 0.5 micro M of NAA (naphtaleneacetic acid) and 4.4 micro M of BAP (6-benzylaminopurine) and MS supplemented with 0.5 micro M of NAA and 2.2 micro M of KIN (kinetin). These shoots and plants were acclimatizated in the following substrates: washed sand, EcoterraReg., coconut fiber, coconut fiber+PlantmaxReg., PlantmaxReg., vermiculite and vermiculite+PlantmaxReg.. The best results for direct acclimatization of shoots and plants of caroa were obtained with EcoterraReg. and PlantmaxReg. substrates. On the other hand, the results showed the necessity to make a study on production cost of these in vitro plants, considering the different situations presented in this work.