Array of Single-cell Pairs on a Microwell Array Based on Positive Dielectrophoresis

摘要

We developed a simple device with 400 (20 × 20) micro-wells that integrated the cell positioning and cell pairing of two different types of cells based on positive dielectrophoretic manipulation. We fabricated a star-shaped micropole array on a conductive substrate and used the areas encircled with micro-poles as microwells for positioning the cells. The different types of cells were successively trapped to form vertical cell pairs in the microwells. The time required for the formation of the array of cell pairs was as short as 1 min.