Detection of L-alanylaminopeptidase activity in microorganisms using fluorogenic self-immolative enzyme substrates

摘要

A series of fluorogenic enzymatic substrates that incorporate a self-immolative spacer were synthesised for the purpose of identifying L-alanylaminopeptidase activity in microorganisms in agar media. These substrates resulted in the generation of fluorescent microorganism colonies with Gram-negative microorganisms. (C) 2016 Elsevier Ltd. All rights reserved.