Effect of vegetable oils on obtaining lipid nanocarriers for sea buckthorn extract encapsulation

摘要

The principal aim of the present study was to develop new safe and highly antioxidant nanostructured lipid carriers loaded with sea buckthorn extract. Three vegetable oils — grape seed oil, sea buckthorn oil and St. John's wort oil (Hypericum perforatum oil) — were used as matrix components and the modified high shear homogenization technique has been employed for the synthesis of nanostructured materials. The effect of these oils on the antioxidant and antimicrobial activities of loaded sea buckthorn extract — nanostructured lipid carriers — has also been studied. For this purpose, a combination of two solid lipids: cetyl palmitate with glyceryl stearate and lecithin/block copolymer has been used. The obtained nanostructured lipid carriers have been characterized for the particle size and zeta potential by means of dynamic light scattering measurements. The nano-dimension morphology of loaded nanostructured lipid carriers was confirmed by transmission electron microscopy. Their crystallinity measured by differential scanning calorimetry has revealed a high disordered lipid matrix. The properties of sea-buckthorn-extract-loaded nanoparticles have been evaluated by an appropriate in vitro analysis (chemiluminescence method). The presence of the three vegetable oils influences extensively the antioxidant properties of the developed nano-formulations, as has been demonstrated using the chemiluminescence technique. The antimicrobial activity of the studied nanostructured lipid carriers, analyzed by the diffusion disc method, shows in most of the samples a high efficiency against Escherichia coli bacteria.