IMMUNOMODULATORY EFFECTS OF HUMAN BONE MARROW OR ADIPOSE TISSUE DERIVED MESENCHYMAL STEM CELLS ON T-HELPER CELL CYTOKINE PRODUCTION IN VITRO

摘要

Mesenchymal stem cells (MSC) have been characterized as multipotent cells which are able to differentiate into several mesodermal and nonmesodermal lineage cells and this feature along with their extensive growth and comprehensive immunomodulatory properties establish them as a promising tool for therapeutic applications. Although bone marrow (BM) is the most common MSC source, cells with similar characteristics have been shown to be present in several others adult tissues. Adipose tissue (AT), large quantities from which could be easily attained, represents an attractive alternative to BM in isolating adipose tissue-derived MSC (AT-MSC). BM-MSCs and AT-MSCs share some immunomodulatory properties as they are both not inherently immunogenic and suppress the proliferation of alloantigen- or mitogen-stimulated T-cells. Our purpose was to comparatively examine the ability of BM-MSCs and AT-MSCs to suppress the cytokine production by mitogen-stimulated Th-1- and Th-2-cells. MSCs and peripheral blood mononuclear cells (PBMCs) were cocultured in the presence of phytohaemagglutinin (PHA) and as a result an inhibition of T-cell cytokine (IFN-gamma and IL-4) production was observed. These results further support the potential therapeutic use of hMSCs in immune-mediated disorders.