Inhibitory Effect of Octylcaffeate on Lipopolysaccharide-Induced Nitric Oxide Production in Macrophages

摘要

We have investigated the effect of octylcaffeate on lipopolysaccharide-induced nitric oxide production in a macrophage-derived cell line, RAW 264.7. Octyl-caffeate (0.1, 0.5, 0.8 and 1muM) inhibited the production in a concentration-dependent manner when treated simultaneously with LPS (1mu g/mL) . Treatment of octylcaffeate (1mu M) between 2 h before and 1h after the treatment of LPS was found to be effective, but the protective efficacy of octylcaffeate was diminishted when given before or after this time interval. Octylcaffeate (0.055 0.1,0.5 and 0.8 mu M) also inhibited the protein expression of inducible nitric oxide synthase. These results suggest that octylcaffeate attenuates lipopolysaccharide-stimulated expression of nitric oxide synthase in macrophages which may help to explain the antiinflammatory actions of this compound.