Construction of the broad-host-range promoter-probe vector containing a kanamycin resistant reporter gene.

摘要

The broad-host-range promoter-probe vector which carries the promoterless kanamycin resistance gene as a reporter gene was constructed. The Klebsiella pneumoniae strains harbouring the plasmids in which the nifH promoter regions were inserted into the cloning site upstream of the reporter gene exhibited considerable increases of kanamycin resistance under N2 fixing conditions. These findings suggested the availability of the newly constructed promoter-probe vector to study the promoter regions of nitrogen fixation (nif) genes from N2 fixing bacteria.