Development of a homogeneous immunoassay based on the AlphaLISA method for the detection of chloramphenicol in milk, honey and eggs.

摘要

BACKGROUND: A homogenous light-induced chemiluminescence immunoassay was developed using AlphaLISA technology for the detection of chloramphenicol (CAP). This technology is based on two different kinds of bead, namely light-sensitive donor beads and beads containing chemiluminescers, also called acceptor beads. A competitive CAP AlphaLISA method was established using artificial antigen-coated acceptor beads, polyclonal antibodies, biotinylated goat anti-rabbit IgG and streptavidin-coated donor beads. RESULTS: The sensitivity of detection was 0.0086 ng mL--1 and the working range was from 0.0096 to 25 ng mL--1. The intra- and inter-assay coefficients of variation were both below 10%. The average recovery rates at spiked levels of 0.05-10 ng mL--1 were 103.2, 108.4 and 91.6% for milk, honey and eggs respectively. The data obtained from the samples showed good correlation with ELISA results. CONCLUSION: The CAP AlphaLISA method is highly sensitive, specific and rapid and is suitable for screening large quantities of samples. Copyright copyright 2012 Society of Chemical Industry