Fractionation of exopeptidase from viscera of Argentina shortfin squid, Illex argentinus.

摘要

For the effective use of exopeptidase from squid viscera as food processing aids, the viscera of the Argentinean shortfin squid (Illex argentinus) were fractionated by various methods, including acetone, ammonium sulfate, anion exchange chromatography and gel filtration. The specific activities of fractions with exopeptidase activity against substrates were higher with LeuPNA than ArgPNA. Total activity and recovery of positive fractions by gel filtration against LeuPNA were 1867 U and 30.69%, respectively, which were the highest among the fractions with exopeptidase activity. Results suggest that gel filtration chromatography was the most efficient method for the fractionation of exopeptidase from the viscera of squid.