A capillary electrophoresis end-column amperometric detection system incorporating disposable copper-plated screen-printed carbon electrodes

摘要

We report here the development of copper-plated screen-printed carbon electrodes (designated as Cu-SPE) to employ as electrochemical detectors for the determination of sugars by capillary electrophoresis (CE). A simple end-column amperometric detection system with easily exchangeable (or even disposable) electrode and capillary in CE is described in this study. A complex alignment procedure was not required in this system based on the end-column electrode arrangement using an 85 cm length and 20 pm (i.d.) capillary. The optimized separation voltage and applied potential were 9 KV and 0.4 V (vs. Ag/AgCl), respectively, for the detection of sugars using the Cu-SPE. Good resolution was obtained by this proposed system with migration times of 28.8, 29.5, 29.9, 30.7, 31.2, and 32.0 min for galactose, glucose, arabinose, fructose, xylose, and ribose, respectively.