Purification and identification of gamma-oryzanol from rice bran

摘要

A physiological active factor - gamma-oryzanol in rice bran was studied. The gamma-oryzanol was separated and identified by thin layer chromatograghy(TLC), reverse-phase HPLC, semipreparative HPLC and electrospray ionization/mass spectrometry(ESI/MS). Its individual components, cycloartenol ferulate and 24-methylene cycloartenol ferulate were obtained. An effective method to purify and separate the gamma-oryzanol is provided. Which is useful for further research on the factional claracteristies of gamma-oryzanol and for the development of new rice bran products.