响应面法优化柱层析工艺提取米糠油皂脚中的谷维素

摘要

以米糠油加工所得的副产物皂脚为原料，用乙酸乙酯索氏抽提得到皂脚浸提物，采用硅胶柱层析的方法对皂脚浸提物分离纯化谷维素，采用单因素和响应面方法进行优化，确定浸提液的谷维素浓度为4.00%，上样量为8.70 g，硅胶用量为60.00 g。结果表明，此工艺得到的谷维素纯度88.5%，皂脚中谷维素的总提取率达到58.7%。%Soapstock,the byproduct of rice bran oil procossing,was used as raw material. Oryzanol was extracted with ethyl acetate from rice bran oil soap stock and purified by silica gel column chromatography methods. Based on single factor experiments and response surface methodology,the optimum conditions were as follows:oryzanol concentration of sample forsilica gelcolumn chromatography was 4.00%,the sample volume was 8.70 g,the amount of silica gel was 60 g. The results showed that the total extraction rate of oryzanol could reached 58.7% while oryzanol purity was 88.5%.