Use of recombinant human thyroid-stimulating hormone for evaluation of thyroid function in guinea pigs [Cavia porcellus)

摘要

Objective—To evaluate the effects of administration of recombinant human (rh) thyroid-stimulating hormone (TSH) for evaluation of thyroid function in euthyroid guinea pigs (Cavia porcellus). Design—Prospective, experimental study. Animals—10 healthy, sexually intact, pet guinea pigs (approx 1 year of age). Procedures—Guinea pigs were given rhTSH (100 mu g, IM); plasma thyroxine concentrations were determined prior to and 3 and 4 hours after rhTSH injection. The animals were housed in 2 groups onthe basis of sex and fed different commercial maintenance diets according to their husbandry. Results—There was no significant difference in thyroxine concentrations between males and females before or after rhTSH injection. There was also no differencebetween thyroxine concentrations at 3 versus 4 hours after rhTSH injection. There was a significant difference between thyroxine concentrations before (median, 9.05 nmol/L [0.70 mug/dl_]; 10% to 90% range, 7.39 to 16.99 nmol/L [0.57 to 1.32 mu g/dL]) and after (mean ± SD, 23.95 ± 4.2 nmol/L) rhTSH injection. Euthyroid guinea pigs had plasma thyroxine concentrations of at least 7.30 nmol/L (0.57 muj/dL) and an increase of at least 2.6 times prestimulation thyroxine concentrations at 3 or 4 hours afterstimulation. Conclusions and Clinical Relevance—The results suggested that rhTSH administered IM can be used for the TSH stimulation testing in guinea pigs. We suggest thyroxine concentration in a euthyroid guinea pig should at least double 3 to 4 hours after rhTSH injection.