SOLUBILIZATION OF RECOMBINANT HUMAN THYROID-STIMULATING HORMONE RECEPTOR AND MEASUREMENT OF AUTOANTIBODY TO HUMAN THYROID-STIMULATING HORMONE RECEPTOR USING THE SAME

摘要

PROBLEM TO BE SOLVED: To solubilize the subject substance for measuring TSHR autoantibody, etc., by bringing a gene recombination human thyroid-stimulating hormone receptor(TSHR) into contact with a nonionic surfactant of a solubilizer in an aqueous medium. ;SOLUTION: A gene encoding a human thyroid-stimulating hormone receptor, its derivative or its variant is incorporated into a vector capable of expressing the gene in a host, the gene is expressed in the host, a gene product encoded by the gene is produced in the cell membrane of the host, a cell membrane fraction is collected from the host cell to give a gene recombination human thyroid-stimulating hormone receptor. Then the human thyroid-stimulating hormone receptor is brought into contact with a nonionic surfactant as a solubilizer in an aqueous medium to solubilize the recombinant human thyroid-stimulating hormone receptor useful for measuring an anti-human TSHR autoantibody suitable for diagnosing thyropathy such as Graves' disease, etc., observing treatment process, etc.;COPYRIGHT: (C)1999,JPO