Influences of cross- and self-pollination on peroxidase activities, isozymes, and histological localization during 'Sharpblue' blueberry fruit development.

摘要

Five-year old Vaccinium corymbosum cv. Sharpblue plant were self- and cross-pollinated (the latter with cv. O'Neal pollen) to study peroxidase (POD) activity, isoenzyme patterns and histological localization during fruit development. Cross-pollination resulted in larger and earlier-ripening fruits. Activities of soluble and bound POD were very high during fruit growth period I, with peaks 10 and 20 days after self- and cross-pollination, respectively. Activity was much higher for cross-pollinated fruits. During fruit growth period II, POD activities were low in both pollination treatments. During ripening, soluble POD increased, then declined in both treatments. Bound POD activities increased during the colour transition from blue to dark blue, with the increase greater in self-pollinated fruits. Banding patterns of soluble and bound POD isoenzymes and their histological localization varied with pollination treatment as well as fruit developmental stage. During fruit ripening, soluble POD activity appeared to be associated with colour transition from light blue to blue, while bound POD activity appeared to be associated with colour transition from blue to dark blue.