Electrochemical oxidation behavior of 8-azaguanine at graphene-Nafion composite film-modified glassy carbon electrode

摘要

A simple but highly sensitive electrochemical sensor for the determination of 8-azaguanine based on graphene- Nafion nanocomposite film-modified glassy carbon electrode (G-Nafion/GCE) was reported. The electrochemical behaviors of 8-azaguanine at G-Nafion/GCE were investigated by cyclic voltammetry (CV), square wave voltammetry (SWV), chronoamperometry (CA), and chronocoulometry (CC). The results showed that the electrochemical sensor exhibited excellent electrocatalytic activity to 8-azaguanine. 8-Azaguanine can be effectively accumulated at G-Nafion/GCE and produce a sensitive anodic peak, due to the synergetic functions of graphene and Nafion. Under the selected conditions, the modified electrode in pH 1.98 Britton-Robinson buffer solution showed a linear voltammetric response to 8-azaguaninewithin the concentration range of 5.0 × 10~(-8)～3.0 × 10~(-5) mol L~(-1), with the detection limit of 1.0 × 10~(-8) mol L~(-1). And, the method was also applied to detect 8-azaguanine in spiked human urine with wonderful satisfactory results.