Coordination investigation of rhenium with MAG3 using LC-MS and UV spectrometer and the simple radiolabelling process

摘要

Due to the low concentration of Re-188-radiopharmaceuticals, identity confirmation of the complex is generally performed only indirectly by assessment of their retention time on HPLC. Herein, LC-MS and UV spectrometer are utilized to confirm the identity of the Re-mercaptoacetyltriglycine (MAG3) complex. The main peak in LC-MS spectrum coincided with the expected ion mass of the oxorhenium (V)-MAG3 complex. Meanwhile, possibility of generation of the tin complex during the coordination process was excluded. Additionally, the simple method of radiolabelling MAG3 with Re-188 was developed. All the experimental results can confirm the purity and effective specific activity of the corresponding Re-188-MAG3.