EFFECTS OF SOIL SALINITY IN THE GROWTH OF AMBROSIA ARTEMISIIFOLIA BIOTYPES COLLECTED FROM ROADSIDE AND AGRICULTURAL FIELD

摘要

Morphological differences were observed between roadside (R) and agricultural field (F) biotypes of Ambrosia artemisiifolia, in which R-type seedlings were shorter and produced larger and heavier seeds under greenhouse grown conditions. Previous findings indicated that A. artemisiifolia R-types exhibited greater salt tolerance with respect to germination. However, the impact of biotype and salt tolerance on morphological variation has not been investigated in A. artemisiifolia plants. After performing replicated greenhouse experiments with both biotypes, it was shown that salinity level was a critical factor influencing both seedling and mature plant size and this response was dependent upon biotype. The R-type exhibited slight but significant increases in growth at low/mild salinity levels (50-100mM) compared with non-saline conditions, while the F-type exhibited significantly reduced growth at the low/mild salinity levels. The reductions in growth of F-type plants in low/mild salinity were similar to those reductions of R-types observed in non-saline conditions. As both biotypes produced seeds at low/mild salinity levels, we conclude that low/mild salinity affects A. artemisiifolia plant size and overall growth rate, and secondly, certain F-type plants may acclimate to the roadside environment over time by reducing their size while producing larger seed under saline conditions. It is possible that this species may exhibit changes in morphology after several generations of exposure to saline roadside conditions. Toxicity due to salt treatment at high salinity (400mM) was observed in both biotypes, whereas the R-type was more tolerant to both low and high salinity levels with respect to seed germination. Differential A. artemisiifolia growth responses which occur from seed germination to plant maturity may be partially attributed to its ability to tolerate saline soil conditions both under greenhouse and field conditions. This ability to tolerate saline conditions may be especially important in early spring when roadside soils experience increased salinity, caused by de-icing salt treatments applied during the winter season.