Study on the fingerprints of radix paeoniae alba and Shenshao composite injection by HPLC

摘要

A simple, rapid, and reproducible method for the qualification of Radix paeoniae alba raw material and Shenshao composite injection was developed. The fingerprint was established using HPLC techniques. Chromatographic fingerprint analysis was carried out on a ODS analytical column. Acetonitrile-phosphoric acid solution (pH 3.0) in gradient program was used as the mobile phase. The detecting wavelength was 230 nm for Radix paeoniae alba and 210 nm for Shenshao composite injection, respectively. The standard fingerprint chromatograms and the similarity of 10 batches of samples were also established. It was demonstrated that the HPLC fingerprint was a powerful tool to characterize the quality of traditional Chinese medicines.