A Novel solid-phase extraction-hplc method for the analysis of anthocyanin and organic acid composition of finnish cranberry

摘要

Spectrophotometric and liquid chromatographic methods were used to determine anthocyanin and organic acid content and com- position of commercial Finnish cranberries ( llaccinium o.".vcoccos L.). Solid-phase adsorbents were used in the fractionation of juice components prior to chromatographic determination. In the freshly prepared cranberry juice the total amount of anthocyanins was 7.7 mg/l 00 mL juice detemlined spectrophotometrically at 510 nm. The most abundant individual anthocyaninswere cyani- din-3-galactoside ( 19.2%), cyanidin-3-arabinoside (23.1% ), peonidin-3-galactoside (21.5%) and peonidin-3-arabinoside (14.1%), as determined by RP-HPLC. The total titrable acidity was 3 .8g/ I OOg. The main organic acids were citric and malic acids, as determined using an HPLC method. Only a few studies have been conducted on this cran- berry species, but more is known about the composition of species fGccinilIm macrocarpon Ait. However, to our surprise, the anthocyanin composition of the berries used in this study was different from those reported previously.