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Methods without markers for the isolation and analysis of nucleic acids in solid-phase devices
Methods without markers for the isolation and analysis of nucleic acids in solid-phase devices
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机译:没有标记的方法用于固相设备中核酸的分离和分析
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摘要
A method for the isolation and / or analysis of nucleic acid molecules in a solid phase device comprising (i) incubating a sample of nucleic acid with dimethyl adipimidate (DMA) under conditions that allow the formation of an acid complex nucleic with DMA; (ii) contacting the complex of (i) with an aminated surface of the solid phase device; and (iii) isolating the nucleic acid complex with DMA from the surface using an elution solution at high pH and / or analyzing the nucleic acid of the complex.
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