Determination of cetylpyridinium chloride residue in chicken meat by hydrophilic interaction chromatography

摘要

Cetylpyridinium chloride (CPC) is used as a disinfectant in poultry processing plants in the United States due to its strong bactericidal effect on food poisoning bacteria such as Campylobacter jejuni/coli, pathogenic Escherichia coli, and Salmonella species. Although high-performance liquid chromatography (HPLC) methods for detecting CPC have been reported, the difficulties involved in the extraction and accurate detection of the residual agent from chicken meat containing proteins and fat have not been resolved. To develop a simple, rapid, stable, and accurate method for measurement of CPC residue in chicken meat by HPLC, a hydrophilic interaction chromatography (HILIC) column was adopted due to the high polarity of CPC. The residual CPC was extracted using a combination of mid-polarity sorbent and weak cation exchanger cartridge and a methacrylate-styrendivinyl benzene cartridge. The calibration curves were linear within the range 0.25-50 μg/mL CPC. This analytical method showed a correlation coefficient (r2) of 0.9997. Recoveries of CPC were between 83% and 89%, and the limit of quantitation was 0.125 μg/g. This proposed method is rapid, simple, and accurate.