Development and validation of an HPLC method for the simultaneous determination of ten sulfonamide residues in whole egg according to 2002/657/EC

摘要

An HPLC method for the determination of ten sulfonamides in egg is described herein. The separation was achieved on an analytical column Kromasil, C 18 5μm, 250 × 4 mm operated at ambient temperature. The mobile phase, a mixture of 0.1% formic acid as solvent A, acetonitrile as solvent B, and methanol as solvent C was delivered to the analytical column under a gradient program. PDA detection was performed for detection and confirmation of separated analytes with monitoring at 265 nm. The examined sulfonamides were extracted from whole egg, with ethyl acetate. The method was validated according to the Decision 2002/657/EC, determining selectivity, stability, decision limit, detection capability, accuracy, precision, and linearity. Overall recoveries ranged from 92.0-113.7%. All RSD values were lower than 10%. The decision limits CCa ranged from 101.74 to108.42μg/kg, while detection capability CCb ranged from 106.84 to 119.01 mu;g/kg.