Separation of selected bile acids by TLC. IV. Comparison of separation of studied bile acids by the use of cluster analysis

摘要

Selected bile acids such as cholic acid (C), glycocholic acid (GC), glycolithocholic acid (GLC), deoxycholic acid (DC), chenodeoxycholic acid (CDC), glycodeoxycholic acid (GDC), and lithocholic acid (LC) were separated on both, silica gel and a mixture of silica gel 60 and Kieselguhr F-254 by using adsorption thin-layer chromatography. The similar analyses were used to compare the separations of studied bile acids. Both analyses showed that on the plates precoated with silica gel, the biggest problem was to separate glycocholic acid from glycodeoxycholic acid. In the case of the separation on silica gel 60 and Kieselguhr F-254 mixture, the biggest problem was to separate C from GLC. The obtained results indicate that similar analysis can be an alternative method of the estimation of chromatographic separations of studied bile acids.