DIFFERENTIAL MOBILITY SEPARATION (DMS)-BASED SEPARATION OF BILE ACID ISOMERS

摘要

Bile acids are involved in a wide range of biological functions including lipid resorption, immunological functions and metabolic regulation. Through metabolic transformations, isomeric and isobaric variants are generated, which makes the unequivocal identification and quantification of individual chemical species difficult. Here we introduce Differential Mobility Separation (DMS) as a methodology for the separation of bile-acid isomers. DMS is an ion mobility technology which separates molecules based on their dipolar moment. We show DMS separation in conjunction with chromatographic separation (LC-DMS-MS) as well as with direct infusion (DMS-MS). While the combination with chromatographic separation may improve selectivity, the separation power of SelexIon is sufficient for a clear separation of isomers, allowing for infusion-based fast quantification without the need for LC development.