ACCELERATED HIGH RESOLUTION DIFFERENTIAL ION MOBILITY SEPARATIONS USING HYDROGEN CARRIER GAS

摘要

A new carder gas medium for differential or field asymmetric waveform on mobility spectrometry (FAIMS) is disclosed. Separations in this medium generally follow those in He/N₂ mixtures known in the art, but are faster and/or exhibit previously unachievable resolving power, peak capacity, and species resolution. The new medium is resistant to electrical breakdown, readily available, and less expensive than current buffers involving substantial He fractions. Performance gains apply broadly across analyte classes, including peptides and other larger biomolecules, and to ions of various charge states. Improvements are particularly large for larger and singly charged ions with lower mobility in gases.