ACCELERATED HIGH RESOLUTION DIFFERENTIAL ION MOBILITY SEPARATIONS USING HYDROGEN CARRIER GAS

摘要

A new carrier gas medium for differential or field asymmetric waveform ionmobility spectrometry (FAIMS) is disclosed. Separations in this mediumgenerallyfollow those in He/N2 mixtures known in the art, but are faster and/or exhibitpreviously unachievable resolving power, peak capacity, and speciesresolution.The new medium is resistant to electrical breakdown, readily available, andlessexpensive than current buffers involving substantial He fractions. Performancegains apply broadly across analyte classes, including peptides and otherlargerbiomolecules, and to ions of various charge states. Improvements areparticularlylarge for larger and singly charged ions with lower mobility in gases.