Carbohydrate Cluster Microarrays Fabricated on Three-Dimensional Dendrimeric Platforms for Functional Glycomics Exploration

摘要

We reported here a novel, ready-to-use bioarray platform and methodology for construction of sensitivecarbohydrate cluster microarrays. This technology utilizes a three-dimensional (3-D) poly(amidoamine)starburst dendrimer monolayer assembled on glass surface, which is functionalized with terminalaminooxy and hydrazide groups for site-specific coupling of carbohydrates. A wide range of saccharides,including monosaccharides, oligosaccharides and polysaccharides of diverse structures, are applicablefor the 3-D bioarray platform without prior chemical derivatization. The process of carbohydrate couplingis effectively accelerated by microwave radiation energy. The carbohydrate concentration required formicroarray fabrication is substantially reduced using this technology. Importantly, this bioarray platformpresents sugar chains in defined orientation and cluster configurations. It is, thus, uniquely useful forexploration of the structural and conformational diversities of glyco-epitope and their functionalproperties.