Studies on In Vitro Selection and Biochemical Characterization of Salt-tolerant Plants of Nicotiana tabacum L.

摘要

Sodium chloride-tolerant organogenic callus lines of Nicotiana tabacum were developed in vitro on Murashige and Skoog medium supplemented with BA, IAA and different concentrations of NaCl. The maximum shoot bud regeneration was achieved from both tolerant and non-tolerant calluses on MS medium supplemented with 1.0 mg/l BA, 0.1 mg/l IAA with or without NaCl within four weeks of culture. Standard growth parameters such as fresh weight and dry weight of organogenic callus, growth tolerant index and enzyme activity (peroxidase and catalase) were used as indicators of salt.tolerance. Measurements as early as two weeks after the beginning of the treatments did not yield consistent results. However, growth tolerance index in the 4-week after the beginningof treatments yielded significant differences among the non-tolerant and tolerant organogenic callus lines. The regenerated shoots were rooted on half-strength MS basal salts supplemented with 2% sucrose but devoid of growth regulator. The regenerated plants from tolerant callus lines were capable of growing in vitro in presence of 175 mM NaCl. This investigation may help in the selection and characterization of salt tolerance in plant improvement programme.