IN VITRO culture method of transgenic tobacco cell cultures NICOTIANA TABACUM L., containing the gene for interleukin-18 people

摘要

1. A method for cultivating a cell culture in vitro of transgenic tobacco Nicotiana tabacum L., containing the gene of human interleukin-18 comprising obtaining explants, induction of callus formation and subsequent subculturing, characterized in that used as explants seedlings segments derived from the transgenic plant seeds Nicotiana tabacum L., containing a gene of human interleukin-18, for which said seeds are sterilized, transferred to bezgormonalnuyu medium and germinated in vitro under light for 28 days, the seedlings were obtained dissected in segments the size of 0.2-0.3 cm, carry them to hormonal growth medium supplemented with the following (mg / l), and incubated in the dark for 3-4 weeks to form kallusa.2. A method according to claim 1, characterized in that said sterilization is carried out in a solution of the following composition (volume fraction) 3. A method according to claim 1, characterized in that for the subculturing medium using a modified Murashige and Skoog medium supplemented with the following (mg / l): 4. A method according to claim 1, characterized in that the cultivation is maintained at ambient temperature 25-27 ° C at 65-70% humidity.