METHOD OF CULTIVATING IN VITRO CELLULAR CULTURE OF TRANSGENIC TOBACCO NICOTIANA TABACUM L CONTAINING HUMAN INTERLEUKINE-18 GENE

摘要

FIELD: medicine.;SUBSTANCE: seeds of transgenic plants Nicotiana tabacum L. are sterilised, removed on hormone-free feeding medium and germinated in virto on light for 28 days. Obtained acrospires are cut into segments with size 0.2-0.3 cm², removed onto modified feeding medium, which includes 2,4-dichlorophenoxyacetic acid, indolylacetic acid and kinetin. For seed sterilising solution which consists of 96% ethanol, 37% hydrogen peroxide and water in volume ratio 7.5:1:1 is used. Formed callus which contains gene of human interleukin-18 is cultivated in darkness for 21-28 days and multiplied by passing to required quantity.;EFFECT: invention can be used in pharmacology for growing biomass of cellular culture of transgenic tobacco Nicotiana tabacum L.;4 cl, 3 tbl, 1 ex