Comparative evaluation of decalcified and non-decalcified freeze-dried bone allografts in rhesus monkeys. I. Histologic findings.

摘要

BACKGROUND: Controversy exists regarding the relative merits of decalcified (DFDBA) and non-decalcified (FDBA) freeze-dried bone allografts when used in periodontal or other oral surgical procedures. METHODS: Under typical sedation, six rhesus monkeys had nylon mesh cylinders containing either DFDBA or FDBA implanted into surgically created vertical grooves on the facial aspects of all posterior quadrants. Each quadrant received three cylinders containing one type of bone, plus one empty cylinder (E) as negative control, and the full thickness flaps were closed to completely cover the cylinders. Cylinders were retrieved at 1, 2, and 3 months and processed for histologic evaluation. Photomicrographs taken at 8x were randomly evaluated using a histometric grid point counting technique for new bone and old bone within the chambers, and the data were analyzed with analysis of variance plus post-tests. RESULTS: The nylon chambers and their contents were well-tolerated by the tissues. FDBA chambers containedmore new bone and total bone than either the DFDBA or E chambers at all time periods (P <0.05). DFDBA was not statistically significantly different than E at any time period. FDBA had less old bone than DFDBA at 3 months (P<0.05). FDBA and DFDBA had more total bone (grafted plus new) present than E at all time periods (P<0.05). CONCLUSION: These results suggest that FDBA may stimulate earlier, more rapid, and more substantial new bone formation than DFDBA in a monkey jaw defect model system.