Cloning and analysis of full-length cDNA of PumNPR1 gene from Pyrus ussuriensis Maxim.

摘要

A study was conducted to find a new gene resource for molecular breeding of Rosaceae plant disease resistance. Pyrus ussuriensis is used as a starting material to clone the full-length cDNA of NPR1 (nonexpressor of pathogenesis-related gene 1) which is a key regulator in SA (salicylic acid)-mediated systemic acquired resistance (SAR) by homologous cloning and RACE techniques. The length of the cDNA sequence was 1767 bp, the open reading frame was 1761 bp, it coded for 586 amino acids, its pI was 5.58, the relative molecular weight was 65.009 ku, it contained 19 types of amino acids and had full BTB/POZ and ANK domains. The homology of NPR1 gene in GenBank database compared with Pyrus pyrifolia, Arabidopsis thaliana, Nicotiana tabacum, Lycopersicon esculentum, Oryza sativa and Helianthus annuus were 98%, 62%, 68%, 65%, 57% and 63%, respectively. The homology of functional area were 99%, 78%, 82%, 79%, 74%, 77%. This NPR1 gene was considered as a homologous gene of Pyrus ussuriensis, and named PumNPR1.