Second, because trypan specifically stains dead cells We would like to express our appreciation for Dr. Ozturk's comments on our original article [1]. A proper discussion of this novel approach evaluating trypan blue instillation for endometrial staining of morcellated hysterectomy specimens is important for patient safety and diagnostic efficiency and accuracy. The first comment regarding trypan being toxic to living cells is a valid concern. Fortunately, we were able to use VisionBlue (Dutch Ophthalmic USA, Kingston, NH), a sterile solution of trypan blue. Each milliliter of VisionBlue contains 0.6 mg trypan blue, 1.9 mg sodium monohydrogen orthophosphate (Na2HPO4.2H2O), 0.3 mg sodium dihydro-gen orthophosphate (NaH2PO4*2H2O); 8.2 mg sodium chloride (NaCl), and water [2]. Trypan blue dye has been used in numerous surgical settings and in diagnostic evaluations to aid in tissue identification or anatomic evaluation. VisionBlue (trypan blue ophthalmic solution) is a selective tissue staining agent used as a medical aid in cataract surgery.
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