Development of TaqMan Probe-Based Real-Time PCR Method for erm(A),erm(B), and erm(C), Rapid Detection of Macrolide—Lincosamide-Streptogramin B Resistance Genes, from Clinical Isolates

摘要

To achieve more accurate and rapid detection of macrolide-lincosamide–streptogramin B resistance genes, erm(A),erm(B), and erm(C), we developed a TaqMan probe-basedreal-time PCR (Q-PCR) method and compared it withconventional PCR (C-PCR), which is the most widelyusing erm gene identification method. The detection limitof Q-PCR was 5 fg of genomic DNA or 5-8 CFU of bacterialcells of Staphylococcus aureus. The utilization of Q-PCRmight shorten the time to erm detection from 3-4 h toabout 50 min. These data indicated that Q-PCR assayappears to be not only highly sensitive and specific, butalso the most rapid diagnostic method. Therefore, theappropriate application of the Q-PCR assay will permitrapid and accurate identification of erm genes from clinicaland other samples.