Population genetics of two STR loci D2S1346 and D2S1353 in a Han population of Chinese.

摘要

Blood samples were collected from unrelated healthy individuals of the Chinese Han ethnic group in the Chengdu city of Sichuan. Genomic DNA samples were extracted using the Chelex-100 method (1). And polymerase chain reaction (PCR) amplification conditions can be accessed at http://www.legalmed.org/ dna/D2S1346 and D2S1353.htm. The volume of PCR reaction for each locus was 20 muL. The PCR products were analyzed by horizontal nondenaturing polyacrylamide gel electrophoresis with a discontinuous buffer system and then visualized by silver staining (2). Data of population genetics and forensic science of the loci D2S1353 and D2S1346 were analyzed using POWERSTATS program (3). The genotype distributions of the two loci were analyzed for Hardy-Weinberg equilibrium according to Hou's method (4). No deviation from Hardy-Weinberg equilibrium was observed.