Rice necrosis mosaic virus, a fungal transmitted Bymovirus: complete nucleotide sequence of the genomic RNAs and subgrouping of bymoviruses

摘要

The complete nucleotide sequence of Rice necrosis mosaic virus (RNMV) RNA1 was determined to be 7178 nt long with one large open reading frame, potentially encoding a polyprotein of 258 kDa with the features of a typical bymovirus. The nucleotide sequence showed 56 % identity with that of Barley mild mosaic virus (BaMMV) and 46 % with those of Oat mosaic virus, Barley yellow mosaic virus and Wheat yellow mosaic virus. RNA2 was 3579 nt long, encoding a 110-kDa polyprotein, and showed 42 and 35/26 % identity with BaMMV and others, respectively. Phylogenetic analysis showed that bymoviruses were classified into two subgroups.