Infectious in vitro transcripts from a cDNA clone of Tobacco mild green mosaic tobamovirus and its biological activity in host and nonhost plants and in their protoplasts

摘要

We have identified a tobamovirus, isolated from pepper (Capsicum annuum cv. Tosajishi), as a Japanese isolate of Tobacco mild green mosaic virus (TMGMV-J). A cDNA clone of TMGMV-J from which infectious RNA transcripts can be synthesized in vitro wasconstructed. The RNA transcript was infectious to several plant species tested here including C. annuum cv. Tosajishi and Nicotiana benthamiana plants. No viral RNA accumulation was observed in either tomato plants or protoplasts inoculated with transcript or virion RNA, indicating that tomato plants were immune to TMGMV. This is the first report of a biologically active TMGMV cDNA clone and direct evidence of the presence of TMGMV in Japan.