Lipoxygenase inhibitors form Paeonia lactiflora seeds

摘要

Previously, the methanolic extract of Paeonia lacttflora seeds was shown to have strong inhibitory activity against soybean lipoxygenase (SLO). Four phenolic compounds were isolated from the seeds by solvent fractionation, Sephadex LH-20 column chromatography and preparative HPLC, and three of them showed strong SLO inhibition and were characterized as trans-resveratrol, #epsilon#-viniferin and luteolin by UV, IR, ~1H-NMR, ~(13)C-NMR and MS spectrometry, trans-Resveratrol (IC_(50)=l.02 #mu#M), #epsilon# -viniferin (IC_(50)=0.81 #mu#M) and luteolin (IC_(50)=10.01 #mu#M), first found in the above seeds, exhibited a potent SLO inhibitory activity although their activity was lower than that of a well-known lipoxygenase inhibitor, nordihydroguaiaretic acid (NDGA) (IC_(50)=0.57 #mu#M). These results suggest that Paeonia lactiflora seeds, now an unused plant seed, may be developed into useful sources of anti-inflammatory drugs.