2,2'-dithienyl diselenide, an organoselenium compound, elicits antioxidant action and inhibits monoamine oxidase activity in vitro

摘要

Context: Organoselenium compounds have been described as antioxidant and neuroprotective agents.Objective: To evaluate the antioxidant action of 2,2'-dithienyl diselenide (DTDS) and its effects in brain monoamine oxidase (MAO) activity in vitro.Materials and methods: Assays for reactive species (RS), lipid peroxidation, protein oxidation, MAO A and B activities in rat brain homogenate as well as mimetic dehydroascorbate reductase and glutathione S-transferase activities were performed using DTDS (uM range).Results: DTDS was effective in decreasing the levels of RS as well as lipid peroxidation induced by malonate, sodium nitroprusside or FeCI2/EDTA and protein carbonyl in the rat brain homogenate. DTDS elicited dehydroascorbate reductase-like and glutathione S-transferase-like activities. DTDS was effective in inhibiting both MAO-A and MAO-B activities.D/sa/ss/on: The results demonstrated that DTDS is an antioxidant agent with non-selective inhibitory effect on MAO activity.Conclusion: DTDS is a promising molecule to be evaluated in experimental models of neurological diseases. ^g>Selenium, brain, rats