An alternative HPLC method for the quantification of floridoside in salt-stressed cultures of the red alga Grateloupia doryphora

摘要

The floridoside content of the marine red alga Grateloupia doryphora was measured using a new. alternative High Performance Liquid Chromatography method suitable for the analysis of a large number of samples, This assay was to study the quantitative changes in floridoside concentration in G. doryphora cultivated in different salinities for 10 days. Marked changes in floridoside concentration occurred rapidly observed in reponse to osmotic stress, providing evidence of its osmoregulatory function. Interestingly, after a rapid decrease at low salinity and a strong increase at high salinity. the floridoside content then slowly returned to its initial level over the last 3 days of culture. This observation confirms the high physiological capacity of G. doryphora to withstand environmental variations and probably contributes to its actual proliferation along French coasts. [References: 16]