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Prevalence of verotoxin-producing Escherichia coli (VTEC) in slurry, farmyard manure and sewage sludge in France

机译:法国粪便,农家肥料和污水污泥中产生维毒素的大肠杆菌(VTEC)的流行

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Aims: The aims of the present study were to determine VTEC prevalence in manure, slurry and sewage sludge in France and to characterize the VTEC strains isolated (virulence genes and serotype). Methods and Results: Seven hundred and fifty-two samples from 55 farmyard manures, 136 bovine and porcine faeces, 114 slurries, 10 composts, and 437 samples from outflows of sewage wastewater treatment plants were analysed. Twenty-four percent contained isolates which were PCR positive for stx gene. Twenty-one VTEC strains were recovered from positive samples by colony hybridization: 76% of them were positive for stx_2 gene, 33% for stx_1 gene, and 19% for eae gene. One strain belonged to serotype O157:H7 and two others to serogroups O26 and O55, respectively. Conclusions: Some of the VTEC strains isolated from environments in France should be considered as potentially pathogenic for humans. Significance and Impact of the Study: Appropriate handling or use of manure, slurry and sewage sludge is necessary so that contamination of the environment and food by VTEC can be prevented.
机译:目的:本研究的目的是确定法国粪便,泥浆和污水污泥中的VTEC流行度,并鉴定分离出的VTEC菌株(毒力基因和血清型)。方法与结果:分析了55种农家肥,136种牛和猪的粪便,114种浆液,10种堆肥和437种污水处理厂废水中的752种样品。 24%的分离株对stx基因PCR呈阳性。通过菌落杂交从阳性样品中回收了21株VTEC菌株:其中76%的stx_2基因阳性,stx_1基因33%和eae基因阳性。一株属于O157:H7血清型,另外两株属于O26和O55血清型。结论:从法国环境中分离出的一些VTEC菌株应被认为对人类具有潜在的致病性。研究的意义和影响:必须适当处理或使用肥料,淤浆和污水污泥,以防止VTEC对环境和食物的污染。

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