Transgene Transfer to United States Commercial Rice Cultivars via Conventional Breeding Techniques

摘要

Breeding programs can benefit from transfer of a foreign gene from one transgenic plant to commercial cultivars through continuous backcrossing, especially to cultivars in which it is difficult to transfer a foreign gene directly through biotechnology. In this study, two homogeneous transgenic plants, T-28 and T-64 (from Taipei 309), and one homogeneous transgenic plant, N-84 (from Nipponbare), were used as donors of Bar gene. Commercial cultivars, Cypress and Laffite, were used as the recurrent parents. Three to five backcrosses were made using the transgenic plants as donor parents and the commercial cultivars as recurrent parent. The results from selected progeny rows, and two-years of yield tests with selected lines, indicated that the target Bar gene could be transferred to lines from homozygous transformants in 2-3 years of backcrossing, giving lines similar to the recurrent parents in phenotype and yield potential.