A high-performance liquid chromatography-tandem mass spectrometry method for the clinical combination study of carboplatin and anti-tumor agent eribulin mesylate (E7389) in human plasma

摘要

An LC/MS/MS method was developed to quantify carboplatin and eribulin mesylate (E7389) in human plasma and urine. For carboplatin, sample clean-up by protein precipitation and supernatant injection into a Waters Spherisorb (R) S5 SCX column was used. Liquid-phase extraction and reverse-phase chromatography on a Polaris (R) C18 column were used for eribulin. Quantitation involved LC/MS/MS with positive electrospray ionization. Accuracy, precision, linearity, range, specificity, recovery and stability were also evaluated. Both compounds were stable in human plasma (>= 80 days at -80 degrees C), at room temperature (>= 4 h), following three freeze-thaw cycles and in 50/50 methanol/H2O (< 4 degrees C for >= 252 days). (c) 2008 Elsevier B.V. All rights reserved.