Ion-pair reversed-phase high-performance liquid chromatographic separation and determination of ruthenium, rhodium, cobalt and copper as chelates with l-(2-pyridylazo)-2-naphthol-6-sulfonic acid

摘要

Ruthemum(III), rhodium(III), cobalt(II) and coppei(II) as chelates with l-(2-pyridylazo)-2-naphthol-6-sulfonic acid (PAN-6S) were separated by ion-pair reversed-phase high-performance liquid chromatography and detected spectro-photometrically at 580 nm The optimum conditions for the separation and determination of the Ru(III)-, Rh(III)-, Co(II)-and Cu(II)-PAN-6S chelates were investigated. The chelates were separated on a Nucleosil C_(18) column (250X4.6 mm I.D.) using acetonitrile-water (30:70, v/v)containing 001 M acetic acid-sodium acetate buffer (pH 5.0) and 0.01 M. tetrabutylammonium bromide (TBABr) as a mobile phase at a flow-rate of 1 0 ml min~(-1). The detection limits were 2.6 ng for Ru, 1 ng for Rh and 0.3 ng for both Co and Cu.